Identification of Bacteria on Cockroach Feet (Periplaneta americana) in Resident Bay of Palu Permai and Sensitivity Test Against Antibiotics

I Nengah Kundera, Evasari Herman Sapu, Mursito Bialangi

Abstract


Cockroaches (Periplaneta americana) a group of Arthropoda is living inside and outside the home because these animals can cause various diseases and act as vectors as well as hospes.  Cockroaches and other animals that act as vectors are strongly associated with the presence of microorganism pathogen carried on their feet. This study aims to identify the bacteria in Cockroach feet at Teluk Palu Permai residential neighborhood and the sensitivity test against antibiotics. This research is an exploratory laboratory, using a standard of bacteria isolation, Gram staining, Microbact system, and antibiotic sensitivity test. The results of the research were successful in identifying bacteria from Cockroaches, obtained from residential environments bacteria thas is Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Salmonella arizonae, and Proteus mirabilis. The discovery of this bacteria is initial research that is useful for the development of handling infectious diseases transmitted by Cockroach vectors. This study has also found that bacteria experience resistance to the antibiotics Vancomycin, Chloramphenicol and Amoxycillin. While Ciprofloxacin and Ofloxacin antibiotics are still sensitive to Escherichia coli bacteria, Klebsiella pneumoniae, Proteus vulgaris, Salmonella arizonae, and Proteus mirabilis. This research is expected to provide preliminary data on the role of Cockroach as a vector of infectious diseases for humans caused by pathogenic bacteria and prevention efforts in the future.

Keywords


antibiotics, isolation, Periplaneta americana, sensitivity

Full Text:

PDF

References


Abdolmaleki, Z., Mashak, Z., & Safarpoor Dehkordi, F. (2019). Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches. Antimicrobial Resistance and Infection Control, 8(1), 1–14. https://doi.org/10.1186/s13756-019-0505-7

Afe, O. T. I. (2015). Microbiology of Cockroaches – A Public Health Concern. International Journal of Scientific Research, 4(4).

Akter, T., Ahmed, S., & Biswas, R. (2018). Isolation and identification of some gram-negative bacteria from cockroaches collected from hospital, restaurant and slum areas of Dhaka city, Bangladesh. Journal of Biodiversity Conservation and Bioresource Management, 3(2), 63–68. https://doi.org/10.3329/jbcbm.v3i2.36029

Al-fattly, H. H. H. H., & Al-aridhi, H. S. (2014). Antibiotic resistant bacteria associated with the cockroach periplaneta americana in Al- Diwaniya city / Iraq. 2(12), 709–714.

Astiti, P. M. A., Bialangi, M., & Kundera, I. N. (2018). Identification of bacteria on Cockroach feet from Hospital area in Palu city and test of sensitivity to antibiotic. IOP Conference Series: Materials Science and Engineering, 434(1). https://doi.org/10.1088/1757-899X/434/1/012123

Bouamama, L., Sorlozano, A., Laglaoui, A., Lebbadi, M., & Aarab, A. (2010). Antibiotic resistance patterns of bacterial strains isolated from Periplaneta americana and Musca domestica in Tangier , Morocco Original Article Antibiotic resistance patterns of bacterial strains isolated from Periplaneta americana and Musca domestica i. May. https://doi.org/10.3855/jidc.336

Cappuccino, J.G. & Sherman N., 2014. (n.d.). New Features Make the Micro Lab More CLINICAL APPLICATION Gram Staining : The First.

Ejimadu, L.C., Goselle, O.N., Ahmadu, YEntomology, A., & Unit, P. (2015). Specialization of Periplaneta Americana (American Cockroach) and Blattella Germanica (German cockroach) Towards Intestinal Parasites : A Public Health Concern. 10(6), 23–32. https://doi.org/10.9790/3008-10642332

Gomes, T. A. T., Elias, W. P., Scaletsky, I. C. A., Guth, B. E. C., Rodrigues, J. F., Piazza, R. M. F., Ferreira, L. C. S., & Martinez, M. B. (2016). Diarrheagenic Escherichia coli. Brazilian Journal of Microbiology, 47, 3–30. https://doi.org/10.1016/j.bjm.2016.10.015

Hammad, K. M., & Mahdy, H. M. (2012). Antibiotic resistant-bacteria associated with the cockroach, Periplaneta americana collected from different habitat in Egypt. New York Science Journal N Y Sci J, 55(1212), 198–206. http://www.sciencepub.net/newyork%0Ahttp://www.sciencepub.net/newyork.

Hasanah, U., Riwayati, R., Idramsa, I., & Prasetya, E. (2017). Molecular Identification of Endophytic Fungi from Bark of Raru (Cotylelobium melanoxylon) that Produce the Antibacterial Compounds. Biosaintifika: Journal of Biology & Biology Education, 9(3), 380. https://doi.org/10.15294/biosaintifika.v9i3.9424

Islam, A., Nath, A. D., Islam, K., Islam, S., Chakma, S., & Hossain, M. B. (2016). Isolation , identification and antimicrobial resistance profile of Staphylococcus aureus in Cockroaches (Periplaneta americana). 7710(September), 1–8.

Kassiri, H., Kasiri, A., & Quaderi, A. (2014). Detection and Prevalence Rate of American Cockroaches (Periplaneta americana) Bacterial Infections in Human Dwellings. 9(4), 3–5. https://doi.org/10.5812/archcid.18737

Khakim, L., & Rini, C. S. (2018). Identifikasi Eschericia coli dan Salmonella sp. pada air kolam renang candi pari. Journal of m]Medical Laboratory Science Atau Tecnology, 1(2), 84–93. https://ejournal3.undip.ac.id/

Kirby, W., Bauer, A., Sherris, J., & Turk, M. (1966). Antibiotic susceptibility testing by standard single disk method. American Journal of Clinical Pathology, 45, 493–496.

Kundera, I. N., & Bialangi, M. (n.d.). Sensitivitas Bakteri Gram Negatif yang Diisolasi dari Kaki Kecoa (Periplaneta americana) di Area Rumah Sakit dan Perumahan di Kota Palu terhadap Beberapa Antibiotik The Sensitivity of Gram Negative Bacteria Isolated from Cockroach Foot (Periplaneta americana). 15, 709–714.

Lai, K. M. (2017). Are cockroaches an important source of indoor endotoxins? International Journal of Environmental Research and Public Health, 14(1). https://doi.org/10.3390/ijerph14010091

Leão-Vasconcelos, L. S. N. De O., Lima, A. B. M., Costa, D. De M., Rocha-Vilefort, L. O., Oliveira, A. C. A. De, Gonçalves, N. F., Vieira, J. D. G., & Prado-Palos, M. A. (2015). Enterobacteriaceae Isolates From The Oral Cavity Of Workers In A Brazilian Oncology Hospital. Revista Do Instituto de Medicina Tropical de São Paulo, 57(2), 121–127. https://doi.org/10.1590/s0036-46652015000200004

Leshan Wannigama, D., Dwivedi, R., & Zahraei-Ramazani, A. (2013). Prevalence and antibiotic resistance of gram-negative pathogenic bacteria species isolated from periplaneta americana and blattella germanica in Varanasi, India. Journal of Arthropod-Borne Diseases, 8(1), 10–20.

Moges, F., Eshetie, S., Endris, M., Huruy, K., Muluye, D., Feleke, T., Silassie, F. G., Ayalew, G., & Nagappan, R. (2016). Cockroaches as a Source of High Bacterial Pathogens with Multidrug Resistant Strains in Gondar Town , Ethiopia. 2016. https://doi.org/10.1155/2016/2825056

Nedelchev, S., Pilarska, D., Takov, D., & Golemansky, V. (2013). Protozoan and nematode parasites of the American coakroach Periplaneta americana (L.) from Bulgaria. Acta Zoologica Bulgarica, 65(3), 403–408.

Nurcahyo et.al., 2012. (2012). Molecular Detection of Toxoplasmosis Using Specific Primers P30, B1, and rDNA. 13(1), 9–13.

Pechal, J. L., Crippen, T. L., Tarone, A. M., Lewis, A. J., Tomberlin, J. K., & Benbow, M. E. (2013). Microbial community functional change during vertebrate carrion decomposition. PLoS ONE, 8(11). https://doi.org/10.1371/journal.pone.0079035

Sponsored, T., State, K., Journal, P., & Vol, D. S. (2017). Tetfund Sponsored Kwara State Polytechnic Journal of Research and Development Studies Vol. 5. No. 1 June 2017. 5(1), 1–13.

Syed, R., Manzoor, F., Adalat, R., Abdul-sattar, A., & Syed, A. (2014). Laboratory Evaluation of Toxicity of Insecticide Formulations from Different Classes against American Cockroach (Dictyoptera : Blattidae). 8(June), 21–34.

Tatang, R. J. A., Tsila, H. G., & Poné, J. W. (2017). Medically Important Parasites Carried by Cockroaches in Melong Subdivision, Littoral, Cameroon. 2017.

Zacharia, V. M., Manzanillo, P. S., Nair, V. R., Marciano, D. K., Kinch, L. N., Grishin, N. V., Cox, J. S., & Shiloh, M. U. (2013). Cor, a novel carbon monoxide resistance gene, is essential for Mycobacterium tuberculosis pathogenesis. MBio, 4(6), 1–9. https://doi.org/10.1128/mBio.00721-13




DOI: https://doi.org/10.33387/tjp.v9i1.1758

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 I Nengah Kundera, Evasari Herman Sapu, Mursito Bialangi

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.


-------------------------------------------------------------------------------------------------------------------------------------------------------------------

           

        

-------------------------------------------------------------------------------------------------------------------------------------------------------------------
TECHNO: Jurnal Penelitian
Published by: LPPM Universitas Khairun
Addres : Jalan Yusuf Abdurrahman Kampus II Unkhair, Kelurahan Gambesi, 97722 Kecamatan Kota Ternate Selatan, Provinsi Maluku Utara, Email: techno@unkhair.ac.id | URL: http://ejournal.unkhair.ac.id/index.php/Techno 

 


Techno Jurnal Penelitian is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

slot gacor slot gacor hari ini slot gacor 2025 demo slot pg slot gacor slot gacor