Study of Resistance on Orchids Induced Rhizoctonia to ORSV Based on Secondary Metabolite Analysis

Mahfut Mahfut, Widi Aryani, Tundjung Tripeni Handayani, Sri Wahyuningsih


Dendrobium discolor and Phalaenopsis amabilis are in high demand because of their beautiful shape, color, texture, and flower arrangement. The current obstacle to orchid cultivation is infection with Odontoglossum ringspot virus (ORSV). One of the efficient ways to control the infection of this pathogen and does not cause an impact on the environment is to use Rhizoctonia mycorrhizae. Mycorrhizal relationship with plants will form induced resistance. Plants resulting from induced resistance will induce secondary metabolite, namely peroxidase enzymes. This type of secondary metabolites will prevent the growth and development of pathogens and plants become resistant to pathogenic infections. The purpose of this study was to determine differences in peroxidase enzyme activity and differences in resistance to P. amabilis and D. discolor after Rhizoctonia induction against ORSV infection. This research was carried out in several stages, namely plantlet preparation, mycorrhizal inoculation, mycorrhizal inoculation, and peroxidase enzyme activity analysis using a UV Vis spectrometer at a wavelength of 420 nm. The results showed that D. discolor had a higher peroxidase enzyme activity than P. amabilis. In the treatment application factor, the peroxidase enzyme activity was the highest in the Mycorrhizal Virus treatment compared to the Mycorrhizal and Virus treatments. This study showed that D. discolor was more resistant to ORSV infection than P. amabilis. and the application of Mycorrhizae proved to be successful in increasing the activity of the peroxidase enzyme as a response to plant protection against viral infections.


Dendrobium discolor, ORSV, Peroxidase Enzyme, Phalaenopsis amabilis, Rhizoctonia

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Anggreiny, A., Handayani, T. T., Mahfut, M., & Wahyuningsih, S. (2021). Study of Orchid Resistance Induced by Ceratorhiza sp. Against ORSV Infection Based on Peroxidase Activity. Bioscience, 5(2), 102-110.

Cho, A. R., Choi, S. H., & Kim, Y. J. (2020). Flowering and photosynthetic responses of Phalaenopsis under elevated CO2 and nutrient supply. Hortic. Sci. Technol, 38, 595-607.

Delyani, R., Normagiat, S., & Apindiati, R. K. (2019). The Existence and Characterization of Mycorrhiza Rhizoctonia sp. Binucleate in Traditional Medicinal Orchid (Plocoglottis Lowii Rchb. F.) from Sanggau Malay Ethnic West Kalimantan. Gontor AGROTECH Science Journal, 5(1), 49-71.

Diez, M. C., Osorio, N. W., & Moreno, F. (2016). Effect of Dose and Type of Fertilizer on Flowering and Fruiting of Vanilla Plants. Journal of Plant Nutrition, 39(9), 1297-1310.

El-Mougy, N. S., Abdel-Kader, M. M., Lashin, S. M., & AA, M. (2013). Fungicides Alternatives as Plant Resistance Inducers Againts Foliar Diseases Incidence of Some Vegetables Ggrown Under Plastics Houses Conditions. International Journal of Engineering and Innovative Technology. 3(16): 71-81.

Ferdhiani, A. A., Lestari, S., & Proklamasiningsih, E. (2015). Aktivitas Enzim Peroksidase dan Kadar Klorofil pada Daun Angsana (Pterocarpus indicus) sebagai Jalan yang Terpapar Timbal. Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal, 32(2), 126-133.

Firgiyanto, R., Aziz, S. A., & Sukma, D. (2016). Uji Ketahanan Anggrek Hibrida Phalaenopsis terhadap Penyakit Busuk Lunak yang Disebabkan oleh Dickeya dadantii. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 44(2), 204-210.

Isharnani, C. E., Nurcahyani, E., & Lande, M. L. (2015). Kandungan Klorofil Daun Planlet Anggrek Tanah (Spathoglottis plicata Blume.) Hasil Pengimbasan Ketahanan terhadap Asam Fusarat secara In Vitro. In Prosiding Seminar Nasional Swasembada Pangan Polinela Bandar Lampung (pp. 86-92). Politeknik Negeri Bandar Lampung.

Kaur, S. (2020). Mycorrhiza in Orchids. Orchids Phytochemistry, Biology and Horticulture: Fundamentals and Applications, 1-14.

Mahfut, M., Daryono, B. S., Joko, T., & Somowiyarjo, S. Survei Odontoglossum Ringspot Virus (ORSV) yang Menginfeksi Anggrek Alam Tropis di Indonesia. Jurnal Perlindungan Tanaman Indonesia, 20(1), 1-6.

Mahfut, M., Daryono, B. S., & Somowiyarjo, S. (2017). Deteksi Odontoglossum ringspot virus pada Anggrek Asli Koleksi Kebun Raya di Indonesia. Jurnal Fitopatologi Indonesia, 13(1), 1-8.

Nnamchi, C. I., Onyetugo, C. A., & Amarachi, I. N. (2021). Peroxidase, an Example of Enzymes with Numerous Applications. Asian Journal of Chemical Sciences, 10(2), 11-22.

Noort, F. V., & Dueck, T. (2015). Influence of Fertilization and A High Daily Light Integral on The Growth and Flowering of Phalaenopsis. ISHS Acta Horticulturae 1078, 1-12.

Putra, R.P. 2021. Identifikasi Jenis Tanaman Anggrek melalui Tekstur Bunga dengan Tapis Gabor dan M-SVM. Journal of Information Technology and Computer Science. 6(1): 29-34.

Sari, V. D. A., Mahfut, M., Wahyuningsih, S., & Tundjung, T. H. (2021). Ceratorhiza Induction towards ORSV Infection on Viability of Phalaenopsis and Dendrobium. Techno: Jurnal Penelitian, 10(1). 54-66.

Shen, R. S., Liao, Y. H., & Huang, K. L. (2018). Orchid cultivation in Taiwan: Conventional and innovative methods. Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols, 427-446.

Soelistijono, R. (2015). Kajian Efektifitas Rhizoctonia SP Mikoriza Dataran Rendah dan Sedang pada Tingkat Keparahan Penyakit (Dsi) Anggrek Phalaenopsis amabilis terhadap Fusarium sp. Biosaintifika: Journal of Biology & Biology Education, 7(2), 112-119.

Tsulsiyah, B., Farida, T., Sutra, C. L., & Semiarti, E. (2021). Important Role of Mycorrhiza for Seed Germination and Growth of Dendrobium Orchids. J Trop Biodiv Biotech, 6(2), jtbb60805.

Vicuna, D., Malone, R. P., & Dix, P. (2011). Increased tolerance to abiotic stresses in tobacco plants expressing a barley cell wall peroxidase. Journal of Plant Sciences, 6(1), 1-13.

Wang, Y. T., & Chang, Y. C. A. (2017). Effects of Nitrogen and The Various Forms of Nitrogen on Phalaenopsis Orchid—A Review. HortTechnology, 27(2), 144-149.



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