KARAKTERISASI KITOSAN CANGKANG LANDAK LAUT JENIS Tripneustes Gratilla DENGAN DEASETILASI KONSENTRASI NaOH YANG BERBEDA

Pratiwi Hadi, Syamsul Bahri, Hamidin Rasulu

Sari


The population of sea urchin Tripneustes gratilla in Tidore City, North Maluku Province, is very abundant and rich in chitosan sources. This variety of sea urchin can be used as a food source. Waste from sea urchin shells has not been utilized properly and efficiently, so one effective alternative that can be used to handle piles of shell waste is to process the chitin content into chitosan. This research aims to determine the characteristics of sea urchin shell chitosan from the use of different NaOH concentrations in the deacetylation process. This research method used a one-factor Completely Randomized Design (CRD) with 5 different NaOH concentration treatments at a heating time of 90 minutes, a temperature of 105oC and 3 repetitions, so that 15 experimental units were obtained. The data obtained were analyzed using the ANOVA test and if the differences showed results that had a real effect then a Least Significant Difference (BNT) further test was carried out at the α = 5% level. The results of the study showed that the effect of adding different concentrations of NaOH had a significant effect on the physical characteristics in the form of yield, color. (L a b), molecular weight, and degree of deacetylation. Chemical characteristics of water content, ash content, fat content and protein content. The characteristics of chitosan analyzed by FTIR showed a high deacetylation degree value of ˃ 80%, SEM morphology showed that each treatment had a mixed form, and the XRD results of chitosan had a crystalline structure with the main peak at an angle of 20 = 19o – 24o with the highest angle aimed at 20 = 24o . If seen from the ranking results of the best treatment, namely C5 (NaOH concentration 18%) with a physical properties yield value of 60.16%, L color 60.16, a value 90.23 and b value 5.91, molecular weight 197.00 kDa, and degree of deacetylation 97.80%, chemical properties include content water 10.19%, ash content 1.17%, fat content 0.48%, and protein content 2.58%.

 

Keywords: chitosan, NaOH, Tripneustes gratilla, deacetylation


Teks Lengkap:

DOWNLOAD PDF 17-27

Referensi


(EFSA), E. F. S. A. (2010). Scientific Opinion on the safety 0f ‘Chitin, glucan’ as a Novel Food. EFSA Journal, 8 (7), 1-17. https://doi.org/ 10.2903/j/efsa.2010.1687. Availabel

Agustina, S., Swantara, I. M. D., & Suartha, I. N. (2015). Isolasi kitin, karakterisasi, dan sintesis kitosan dari kulit udang. Jurnal Kimia, 9(2), 271-278.

Akerina, Febrin. O.,2015. Eksplorasi Senyawa Antimikroba Dan Antioksidan Dari Bulu Babi (Diadema Setosum), [Skripsi], Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor. Bogor.

Albaar N, Popa R. F, Wulansari A, and Rasulu H., 2022. Characterization of Chitosan Waste Shells of Sea Urchins Type Diadema Cetosum from the Waters of Ternate City. Proceeding IOP, International Conference Marine and Ficeries. Khairun University. Ternate. Indonesia.

Angka SL, Suhartono MT. 2000. Bioteknologi Hasil Laut. Bogor:PKSPL, Institut Pertanian Bogor.

Aprilia, D. (2015). Potensi Kitosan sebagai Agen Antioksidatif pada Hepar yang Diinduksi Plumbum. Jurnal Majority, 4(8), 85-88.

Br Silaban, B., & Srimariana, E. S. 2013. Kandungan nutrisi dan pemanfaatan gonad bulu babi (Echinothrixs calamaris) dalam pembuatan kue bluder. JurnalPengolahan Hasil Perikanan Indonesia, 16 (2).

Cahyono, E. (2018). Karakteristik kitosan dari limbah cangkang udang windu (Panaeus monodon). Akuatika Indonesia, 3(2), 96-102.

Cahyono, E., Jonas, J.F., Lalenoh, B.A., Kota, N. 2019. Karakteristik Kalsium Karbonat (CaCO3) Dari Cangkang Landak Laut (Diadema setosum). Jurnal FishtecH. 8(1): 27-33.

Cahyono, E., Wodi, S. I. M., & Kota, N. K. (2018). Aplikasi kitosan kulit udang windu (panaeus monodon) sebagai pengawet alami pada tahu. Jurnal Ilmiah Tindalung, 4(1), 41-44.

Chasana E, Andamari R. 2011. Komposisi Kimia, ProfilAsamAemak dan Asam Amino gonad Bulu Babi Tripneustes gratilla dan Salmanicsp dan Potensi Pengmbangannya. Di dalam prosiding seminar kelautan LIPI-UNHAS ke 1. Bali sumber daya Laut, puslitbang Oseanologi-LIPI. 269-274.

Fadli, A., Drastinawati, D., Alexander, O., & Huda, F. (2018). Pengaruh rasio massa kitin/naoh dan waktu reaksi terhadap karakteristik kitosan yang disintesis dari limbah industri udang kering. Jurnal sains materi Indonesia, 18(2), 61..

Fajri, F., Thaib, A., & Handayani, L. (2019). Penambahan mineral kalsium dari cangkang kepiting bakau (Scylla serrata) pada pakan terhadap pertumbuhan dan kelangsungan hidup udang galah (Macrobrachium rosenbergii). Depik, 8(3), 185-192.

Fajri, R., & Amri, Y. (2018). Uji Kandungan kitosan dari limbah cangkang tiram (Crassostrea sp.). Jurnal Jeumpa, 5(2), 101-105.

G. Hao, Y. Hu, L. Shi, J. Chen, A. Cui, W. Weng, dan K. Osako,”Karakteristik fisikokimia kitosan dari cangkang rajungan (Portunustritu berculatus) yang dibuat dengan pretreatment air subkritis”. Laporan Ilmiah, 11(1), 1-9. 2021.

Gaspersz, 1991. Metode Perancangan Percobaan. Armico, Bandung

GRAS. 2012. Chitoclear® shrimp-derived chitosan: food usage conditions for general recognition of safety. Iceland (IL): GRAS.

Hadinoto, S., Sukaryono, I. D.Siahay, Y. 2017. Kandungan Gizi Gonad Dan Aktivitas Antibakteri Ekstrak Cangkang Bulu Babi (Diadema setosum). Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 12(1): 71-78.

Hardani, P. T., Sari, D. P., & Rahayu, A. (2021). Isolasi dan Identifikasi Kitosan dari Cangkang Kreca (Bellamya javanica) dengan Spektroskopi Inframerah. FARMASIS: Jurnal Sains Farmasi, 2(2), 36-40

Instruction Manual. 2002. Chroma Mater CR-400/410. Konica Minolta Optics Inc., Japan.

Kumari, S., Rath, P., & Kumar, A. S. H. 2016. Chitosan from shrimp shell (Crangoncrangon) and fish scales (Labeorohita): Extraction and characterization Suneeta. African Journal of Biotechnology, 15(24), 1258-1268.

Kurnia, G. S., Anggraeni, M., Ningsih, M. A. L., Lianastuti, M., Suciyanti, Q. P., & Yuniarsih, N. (2022). Review Artikel: Perbandingan Berbagai Polimer Dan Pengaruhnya Terhadap Penghantaran Sediaan Patch Bukal. Jurnal Pendidikan dan Konseling (JPDK), 4(6), 12056-12065.

Kurniawan, M. F., Fitrilia, T., Nacing, N., & Chosida, H. N. (2023). Pembuatan Label Indikator Ekstrak Secang (Caelsapinia sappan L.) Menggunakan Carrier Jenis Kitosan, Pati, dan Kertas. Jambura Journal of Chemistry, 5(1).

Kusumaningsih, T., Suryanti, V., & Permana, W. (2004). Karakterisasi Kitosan Hasil Deasetilasi Kitin Dari Cangkang Kerang Hijau (Mytilus viridis linneaus). Alchemy Jurnal Penelitian Kimia, 3(1).




DOI: https://doi.org/10.33387/jpk.v2i2.7425

Refbacks

  • Saat ini tidak ada refbacks.




Journal PoliciesSubmissionsPeople
 Information


Editorial Officer
Jl. Jusuf Abdurahman, Gambesi, Ternate, North Maluku - Indonesia
E-mail : hamidinrasulu@yahoo.com

Creative Commons License
Jurnal Pertanian Khairun (JPK) Publisher Magister Ilmu Pertanian, Universitas Khairun This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.