Morphometric and genetic identification of Mackerel (Rastrelliger sp.) collected from Muara Baru Fish Market, Jakarta

Ummu Salma, Hawis Madduppa

Abstract


Ecosystems in Indonesia have a lot of potential resources, and one of them is seafood resources. Mackerel is potential catches of fish in the Jakarta Bay (as part of Java Sea area) that has high economic value. The Java Sea has only two mackerel species, Rastrelliger brachysoma and R. kanagurta which have similar morphology. Therefore in this study was conducted to compare morphometric and molecular analysis (DNA barcoding) using COI sequences so that specific species from specimens could be identified. The analyzed fish samples have striking characteristics such as blackline on the back, a black line near the pectoral fins, and a dark body part is extending above the rib line. The dorsal fins have a yellowish-gray color, yellowish pectoral, and pectoral fins.The result showed that measurements characteristic features of mackerels in Muara Angke belonging to Rastrelliger genera. DNA Barcoding analysis showed that the collected sample identified as Rastrelliger kanagurta with 99.25% identification similarity. The DNA code can distinguish between all fish species and identify the eggs, larvae, and carcass pieces of this species. So that fish in fish landings can be identified and tagged according to their species. This study has successfully identified a sample from Muara Angke by using a DNA code and therefore would be beneficial for managing food controlling and food safety concerns.

 Keywords: molecular identification, DNA barcoding, Rastrelliger kanagurta, morphological analysis 


References


Ardura, A., Planes, S., Garcia-vazquez, E. 2013. Applications of DNA barcoding to fish landing: authentication and diversity assessment. Zookeys 65,49–65. https://doi.org/10.3897/zookeys.365.6409

Bingpeng, X., Heshan, L., Zhilan, Z., Chunguang, W., Yanguo, W., Jianjun, W. 2018. DNA barcoding for identification of fish species in the Taiwan Strait. PLoS ONES One 1–13.

Collette, B.B., Nauen, C.E. (1983). FAO Species Catalogue. Vol. 2. Scombrids of the world. An annotated and illustrated catalogue of tunas, mackerels, bonitos and related species known to date. Rome.

Cren, E.D. Le 1951. The Length-Weight Relationship and Seasonal Cycle in Gonad Weight and Condition in the Perch (Perca fluviatilis). Journal of Animal Ecology 20, 201–219. https://doi.org/10.2307/1540

Faisal, G.A., Azizah, M.N.S., Darlina, M.N. 2012. Utilisation of DNA barcoding for identification of fish products. The Proceedings of The 2nd Annual International Conference Syiah Kuala University 2, 22–24.

Hariati, T., Taufik, M., Zamroni, A. 2005. Beberapa Aspek Reproduksi Ikan Layang (Decapterus russelli) dan Ikan Banyar (Rastrelliger kanagurta) di Perairan Selat Malaka Indonesia [Some Aspects of Reproduction of Decapterus russelli and Rastrelliger kanagurta in the Malacca Strait of Indonesia]. Jurnal Penelitian Perikanan Indonesia 11, 47–56.

Hebert, P.D.N., Cywinska, A., Ball, S.L., Jeremy, R. 2003. Biological identifications through DNA barcodes. Proc. R. Soc. Lond 313–321. https://doi.org/10.1098/rspb.2002.2218

Indaryanto, F.R., Imai, H., Wardiatno, Y. 2015. Genetic variation of short body mackerel, Rastrelliger brachysoma of Jawa Island, Indonesia based on mtDNA control region sequences. AACL Bioflux 8, 648–655.

Karim, A., Iqbal, A., Akhtar, R., Rizwan, M., Amar, A., Qamar, U., Jahan, S. 2015. Barcoding of fresh water fishes from Pakistan. Mitochondrial DNA 00, 1–4. https://doi.org/10.3109/19401736.2015.1043544

Kusuma, A.B., Bengen, D.G., Madduppa, H., Subhan, B., Arafat, D., Negara, B.F.S.P. (2016). Close genetic connectivity of soft coral Sarcophyton trocheliophorum in Indonesia and its implication for marine protected area. Aceh Journal of Animal Science 1, 50–57. https://doi.org/10.13170/ajas.1.2.4867

Luther, G. 1973. Observations on the biology and the fishery of the Indian mackerel, Rastrelliger kanagurta (Cuvier) from Andaman Islands. Indian Journal of Fisheries 20, 425–447.

Madduppa, H., Taurusman, A.A., Subhan, B., Anggraini, N.P., Fadillah, R., Tarman, K. 2017. Short communication: Dna barcoding reveals vulnerable and not evaluated species of sea cucumbers (Holothuroidea and Stichopodidae) from Kepulauan Seribu reefs, Indonesia [Some Aspects of Reproduction of Decapterus russelli and Rastrelliger kanagurta in th. Biodiversitas 18, 893–898. https://doi.org/10.13057/biodiv/d180305

Madduppa, H.H., Bahri, S., Subhan, B., Anggraini, N.P., Ohoiulun, H., Abdillah, T., Arafat, D., Santoso, P., Sangadji, I.M. 2019. DNA barcoding of sea turtles (Dermochelyidae and Cheloniidae) and its protocol using different tissues quality: Implication to conservation managers. IOP Conference Series: Earth and Environmental Science 278. https://doi.org/10.1088/1755-1315/278/1/012041

Ministry of Marine Affairs and Fisheries. 2016. Ministerial Decree No. 47/2016 Concerning Estimation of Fisheries Potential in Indonesian FMAs. https://doi.org/10.1016/j.marpol.2018.01.027

Muchlisin, Z.A., Masazurah, A.R., Talib, A.A., Siti-Azizah, M.N., Samsudin, B., Jamsari, A.F.J. 2009. Genetic identification of four Malaysian mackerel species off Coast of Peninsular Malaysia based on molecular marker. 8th Malaysia genetics Congress 73–77.

Muchlisin, Z.A., Musman, M., Azizah, M.N.S. 2010. Length-weight relationships and condition factors of two threatened fishes, Rasbora tawarensis and Poropuntius tawarensis , endemic to Lake Laut Tawar , Aceh Province , Indonesia Technical contribution Length-weight relationships and condition factors of. Journal of Applied Ichthyology 26, 949–953. https://doi.org/10.1111/j.1439-0426.2010.01524.x

Saanin, H. 1984. Taksonomi dan kunci identifikasi ikan [Fish taxonomy and identification key]. Binacipta, Bogor.

Saleky, D., Setyobudiandi, I., Toha, H.A., Takdir, M., Madduppa, H.H. 2016. Length-weight relationship and population genetic of two marine gastropods species (Turbinidae: Turbo sparverius and Turbo bruneus) in the Bird Seascape Papua, Indonesia. Biodiversitas 17, 208–217. https://doi.org/10.13057/biodiv/d170130

Solanki, H.U., Mankodi, P.C., Nayak, S.R., Somvanshi, V.S. 2005. Evaluation of remote-sensing-based potential fishing zones (PFZs) forecast methodology. Continental Shelf Research 25, 2163–2173. https://doi.org/10.1016/j.csr.2005.08.025

Sujastani, T. 1976. The Species of Rastrelliger in the Jawa Sea, Their Taxonomy and Morphometry (Perciformes, Scombridae). Marine Research in Indonesia 16, 1. https://doi.org/10.14203/mri.v16i0.345

Thu, P.T., Huang, W.C., Chou, T.K., Van Quan, N., Van Chien, P., Li, F., Shao, K.T., Liao, T.Y. 2019. DNA barcoding of coastal ray-finned fishes in Vietnam. PLoS ONE 14. https://doi.org/10.1371/journal.pone.0222631

Toha, A.H., Widodo, N., Subhan, B., Himawan, M.R., Tania, C., Noor, B.A., Stewart, B.S., Madduppa, H.H. 2016. Close genetic relatedness of whale sharks, Rhincodon typus in the Indo-Pacific region. AACL Bioflux 9, 458–465. https://doi.org/10.5339/qproc.2016.iwsc4.31

Triantafyllidis, A., Bobori, D., Koliamitra, C., Mpanti, M., Petriki, O., Karaiskou, N., Triantafyllidis, A., Bobori, D., Koliamitra, C., Triantafyllidis, A., Bobori, D., Koliamitra, C. 2011. DNA barcoding analysis of fish species diversity in four north Greek lakes. Mitochondrial DNA 1736, 37–42. https://doi.org/10.3109/19401736.2010.542242

Vartak, V.R., Narasimmalu, R., Annam, P.K., Singh, D.P., Lakra, W.S. 2014. DNA barcoding detected improper labelling and supersession of crab food served by restaurants in India. Journal Science Food Agriculture. https://doi.org/10.1002/jsfa.6728

Wang, L., Wu, Z., Liu, M., Liu, W., Zhao, W., Liu, H., You, F. 2018. DNA barcoding of marine fish species from Rongcheng Bay, China. PeerJ 1–19. https://doi.org/10.7717/peerj.5013

Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R., Hebert, P.D.N. 2005. DNA barcoding Australia’ss fish species. Philosophical Transactions of The Royal Society B Biological Sciences. https://doi.org/10.1098/rstb.2005.1716

Zargar, U.R., Yousuf, A.R., Mushtaq, B., Jan, D. 2012. Length-Weight Relationship of the Crucian carp, Carassius carassius in Relation to Water Quality, Sex and Season in Some Lentic Water Bodies of Kashmir Himalayas Length – Weight Relationship of the Crucian carp, Carassius carassius in Relation to Water. Turkish Journal of Fisheries and Aquatic Sciences 12, 685–691. https://doi.org/10.4194/1303-2712-v12




DOI: https://doi.org/10.33387/jikk.v4i2.3880

Refbacks

  • There are currently no refbacks.


 

 

 

Address : Marine Science Study Program, Faculty of Fisheries and Marine Science - Khairun University, Ternate, North Maluku, Indonesia

slot gacor slot